Propagation of Piper carniconnectivum through leaf cuttings

Main Article Content

Eric Jonisson Rios Bisi
Leormando Fortunato Dornelas Júnior


The regeneration of roots and shoots through leaf cuttings is a feasible technique for plant clonal propagation, using a quite available organ, which can be collected without great damage to the plant. A protocol of propagation through leaf cuttings was defined to Piper carniconnectivum, a plant whose compounds have great potential use in medicine and agriculture. Leaves were cut in halves (apical and petiolar) by transverse cutting in the middle of the leaf blade. The petiolar parts were immersed into a solution of indole 3-butyric acid (IBA) at 1000 ppm for 30 seconds, or not submitted to the hormone. Then the cuttings were planted in soil, using two positions of the petiolar halves: petiole down and petiole up. A factorial design was used – 2 times of immersion in IBA x 2 leaf half position x 3 blocs x 6 replications. After 145 days the number of shoots, shoot length, leaf area, dry matter of aerial part, root volume and root dry matter were evaluated. The highest number of shoots was observed in the petiole down position without IBA. In relation to the other aerial characteristics – shoot length, leaf area and dry matter of the aerial part, the highest values were observed both in the petiole down position without IBA and in the petiole up position with immersion in IBA. The characteristics related to the root – root volume and root dry matter were both highest in the petiole up position with immersion in IBA. Leaf cuttings can be a practical method to propagate P. carniconnectivum vegetatively. Both petiole up cutting with immersion in IBA and petiole down cutting without immersion in hormone can be used as propagules.


Download data is not yet available.


Metrics Loading ...

Article Details

How to Cite
ALVES DOS SANTOS, M. R., Bisi, E. J. R., & Dornelas Júnior, L. F. (2019). Propagation of Piper carniconnectivum through leaf cuttings. International Journal for Innovation Education and Research, 7(10), 15-20.


Abbasi BH, Ahmad N, Fazal H and Mahmood T, 2010. Conventional and modern propagation techniques in Piper nigrum. Journal of Medicinal Plants Research 4(1): 7-12.
Ahmad N, Abbasi BH, Fazal H, Khan MA and Afridi MS, 2014. Effect of reverse photoperiod on in vitro regeneration and piperine production in Piper nigrum L. Comptes Rendus Biologies, 337: 19-28.
Ahmad N, Fazal H, Abbasi BH, Rashid M, Mahmood T and Fatima N, 2010. Efficient regeneration and antioxidant potential in regenerated tissues of Piper nigrum L. Plant Cell, Tissue and Organ Culture, 102: 129-134.
Ahmad N, Guo B, Fazal H, Abbasi BH, Liu CZ, Mahmood T and Shinwari ZK, 2011. Feasible plant regeneration in black pepper from petiole explants. Journal of Medicinal Plants Research 5(18): 4590-4595.
Balbuena TS, Santa-Catarina C, Silveira V, Kato MJ and Floh EIS, 2009. In vitro morphogenesis and cell suspension culture establishment in Piper solmsianum C. DC. (Piperaceae). Acta Botanica Brasilica, 23(1): 274-281.
Basak UC, Dash D, Jena GJP and Mahapatra AK, 2014. New technique for adventitious rooting and clonal propagation of Piper longum L. (pippali) through leaf cuttings. African Journal of Plant Science, 8(2): 108-112.
Bhat SR, Chandel KPS, and Malik SK, 1995. Plant regeneration from various explants of cultivated Piper species. Plant Cell Reports, 14:398-402.
Bhat SR, Kackar A and Chandel KPS, 1992. Plant regeneration from callus cultures of Piper Iongum L. by organogenesis. Plant Cell Reports, 11:525-528.
Danelutte AP, Constantin MB, Delgado GE, Braz-Filho R and Kato MJ, 2006. Divergence of secondary metabolism in cell suspension cultures and differentiated plants of Piper cernuum and P. crassinervium. Journal of the Brazilian Chemical Society, 16(6b): 1425-1430.
Dyer LA, Richards J and Dodson C., 2004. Isolation, synthesis, and evolutionary ecology of Piper amides. In: Dyer LA and Palmer ADN (Eds.) Piper: a model genus for studies of phytochemistry, ecology, and evolution. New York: Kluwer Academic.
Dornelas Jr LF, Bisi EJR and Santos MRA, 2018. Propagation of Piper hispidum through leaf cuttings. International Journal of Development Research, 8(8): 22414-22418.
Freitas GC, Batista Jr JM, Franchi Jr GC, Nowill AE, Yamaguchi LF, Vilcachagua JD, Favaro DC, Furlan M, Guimarães EF, Jeffrey CS and Kato MJ, 2014. Cytotoxic non-aromatic B-ring flavanones from Piper carniconnectivum C. DC. Phytochemistry, 97: 81–87.
Haissig BE, 1986. Metabolic processes in adventitious rooting of cuttings. In: Jackson MB. New root formation in plants and cuttings. Dordrecht: Martinus Nijhoff Publishers.
Issa Y, Watts DC, Duxbury AJ, Brunton PA, Watson MB and Waters CM, 2003. Mercuric chloride: toxicity and apoptosis in a human oligodendroglial cell line MO3.13. Biomaterials, 24: 981-987.
Kelkar SM, Deboo GB and Krishnamurthy KV, 1996. In vitro plant regeneration from leaf callus in Piper colubrinum Link. Plant Cell Reports, 16: 215-218.
Magevski GC, Czepak MP, Schmildt ER, Alexandre RS and Fernandes AA, 2011. Propagação vegetativa de espécies silvestres do gênero Piper, com potencial para uso como porta enxertos em pimenta-do-reino (Piper nigrum). Revista Brasileira de Plantas Medicinais, 13: 559-563.
Maju TT and Soniya EV, 2012. In vitro regeneration system for multiplication and transformation in Piper nigrum L. International Journal of Medicinal and Aromatic Plants, 2(1): 178-184.
Mercier H, 2008. Auxinas. In: Kerbauy GB. Fisiologia Vegetal. 2.ed. Rio de Janeiro: Guanabara Koogan.
Micaroni RCCM, Bueno MIMS, Jardim WF, 2000. Mercury compounds: review on determination, treatment and disposal methods. Química Nova, 23(4): 487-495.
Michel JL, Chenb Y, Zhang H, Huang Y, Krunic A, Orjala J, Veliz M, Soni KK, Soejarto DD, Caceres A, Perez A and Mahady GB, 2010. Estrogenic and serotonergic butenolides from the leaves of Piper hispidum Swingle (Piperaceae). Journal of Ethnopharmacology, 129: 220–226.
Monteiro D, 2018. Flora of the canga of the Serra dos Carajás, Pará, Brazil: Piperaceae. Rodriguésia 69(3): 1285-1309.
Navickiene HMD, Bolzani VS, Kato MJ, Pereira MS, Bertoni BW, França SC and Furlan M, 2003. Quantitative determination of anti-fungal and insecticide amides in adult plants, plantlets and callus from Piper tuberculatum by reverse-phase high-performance liquid chromatography. Phytochemical Analysis, 14(5): 281-284.
Padham B, 2015. Regeneration of plantlets of Piper longum L. through in vitro culture from nodal segments. Journal of Applied Biology and Biotechnology, 3(05): 035-039.
Pandey S, Kumar R, Sharma S, Nagpure NS, Srivastava SK and Verma MS, 2005. Acute toxicity bioassays of mercuric chloride and malathion on air-breathing fish Channa punctatus (Bloch). Ecotoxicology and Environmental Safety, 61: 114-120.
Rani D and Dantu PK, 2012. Direct shoot regeneration from nodal, internodal and petiolar segments of Piper longum L. and in vitro conservation of indexed plantlets. Plant Cell, Tissue and Organ Culture, 109: 9-17.
Rao MV and Sharma PSN, 2001. Protective effect of vitamin E against mercuric chloride reproductive toxicity in male mice. Reproductive Toxicology, 15: 705-712.
Raven PH, Evert RF and Eichhorn SE, 2007. O sistema caulinar: estrutura primária e desenvolvimento. In: ___. Biologia Vegetal. 5.ed. Rio de Janeiro: Guanabara Koogan.
Umadevi P, Saji KV and Suraby EJ, 2015. Meristem culture for rapid regeneration in Black pepper (Piper nigrum Linn.). Annals of Plant Sciences, 4(03): 1029-1032.
Verstraeten I, Beeckman T and Geelen D, 2013. Adventitious Root Induction in Arabidopsis thaliana as a model for in vitro root organogenesis. In: Smet, I.D. Plant Organogenesis: methods and protocols. New York: Springer.
Zhang Z, Zhao L, Chen X and Zheng X, 2008. Successful micropropagation protocol of Piper methysticum. Biologia Plantarum, 52(1): 110-112.

DB Error: Unknown column 'Array' in 'where clause'