Cytogenetic evaluation of α, β-amirina obtained in resin of Protium heptaphylum (Aubl.) Marchand in polychromatics erythrocytes of mice swiss.

Authors

  • Lucas Rodrigues do Rego Federal University of Amapá image/svg+xml
  • Everton Pantoja Vale Federal University of Amapá image/svg+xml
  • Danilo Dheyvison Nascimento Pureza Federal University of Amapá image/svg+xml
  • Moacir de Azevedo Bentes Monteiro Neto Federal University of Amapá image/svg+xml
  • Fernando Antônio de Medeiros Federal University of Amapá image/svg+xml
  • Rebecca Martins

DOI:

https://doi.org/10.31686/ijier.vol9.iss4.3007

Keywords:

Almecegueira, α, β-amirine, Amazon Rainforest

Abstract

The Amazon Rainforest has a great variety of medicinal plants, among them we can highlight the “Almecegueira” or “Breu Branco” (Protium heptaphylum) in Portuguese, the producer of a greenish-white resin that hardens when it touches the air, known by its gastroprotective and anti-inflammatory effects. These effects are attributed to a triterpene mixture of α and β amirine, predominant in the resin. The purpose of the study is to obtain a cytogenetic profile to the α, β-amirine mixture obtained in the resin of P. heptaphylum. For this, the micronucleus test was used in peripheral blood and bone marrow; administering solution in Swiss mice with the dosages of 1mg/Kg, 3mg/Kg, and 10mg/Kg, diluted in 5% DMSO, the effects were observed in 24h and 48h after the treatment. For the test in peripheral blood the mice’s caudal vein was punctured, while for the bone marrow test, the femurs of the animals were obtained from which bone marrow samples were taken. It was found that in peripheral blood, the administration of the compounds did not cause genotoxicity in 24h and 48h, in contrast, antigenotoxicity was, for concentrations 1; 3 and 10mg/kg, respectively 10%; 12%; 67% in 24h and 9%; 15%; 73% in 48h.  In the bone marrow, no genotoxicity was observed, as for antigenotoxicity was observed that for concentrations 1; 3 e 10mg/kg the percentage of reduction was respectively: 11%, 15%, and 30% in 24h and 13% 16% 33% in 48h. It is concluded that the studied compound can be an alternative for treatments in the future since it presents low toxicity and high antigenotoxic potential.

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Author Biographies

Lucas Rodrigues do Rego, Federal University of Amapá

Genetic and Biomolecular Research Group (GPCBIO), Department of Biological Sciences and Health

Everton Pantoja Vale, Federal University of Amapá

Genetic and Biomolecular Research Group (GPCBIO), Department of Biological Sciences and Health

Danilo Dheyvison Nascimento Pureza, Federal University of Amapá

Genetic and Biomolecular Research Group (GPCBIO), Department of Biological Sciences and Health

Moacir de Azevedo Bentes Monteiro Neto, Federal University of Amapá

Genetic and Biomolecular Research Group (GPCBIO), Department of Biological Sciences and Health

Fernando Antônio de Medeiros, Federal University of Amapá

Genetic and Biomolecular Research Group (GPCBIO), Department of Biological Sciences and Health

References

Alves, J.M., Munari, C.C., De Azevedo Bentes Monteiro Neto, M., Furtado, R.A., Senedese, J.M., Bastos, J.K., Tavares, D.C., 2013. In vivo protective effect of Copaifera langsdorffii hydroalcoholic extract on micronuclei induction by doxorubicin. Journal of Applied Toxicology 33, 854-860. https://doi.org/10.1002/jat.2777

Aparecida R.F., De Andrade B.C.A.M., Da Silva F.M.C., kato, F.H., Cunha, W.R, Tavares, D.C. 2006. Antimutagenicity of ursolic acid and oleanolic acid against doxorubicin-induced clastogenesis in balb/c mice. Life sciences, 1268–1273. doi:10.1016/j.lfs.2006.03.038

Aragao, G.F., Pinheiro, M.C.C., Bandeira, P.N., Lemos, T.L.G., Viana, G.S.B. 2002: Efeito Antiinflamatório da Fração Isomérica de α.- e β.-amirina Isolada de Protium heptaphyllum. (aubl) march. XVII. Simpósio de Plantas Medicinais do Brasil.

Aragao, G.F., Pinheiro, M.C.C., Bandeira, P.N., Lemos, T.L.G., Viana, G.S.B. 2008. Analgesic And Anti-Inflammatory Activities Of The Isomeric Mixture Of Alpha- And Beta-Amyrin Fromprotium Heptaphyllum (Aubl.) March. Journal Of Herbal Pharmacotherapy, 31–47. Doi:10.1080/J157v07n02_03

Araujo, D.A., Takayama, C., de-Faria, F.M., Socca, E.A., Dunder, R.J., Manzo, L.P., Souza-Brito, A.R. 2011. Gastroprotective effects of essential oil from Protium heptaphyllum on experimental gastric ulcer models in rats. Revista Brasileira de Farmacognosia, 721-729.

Bandeira, P.N., Lemos, T.L., costa, S.M., Santos, H.S. 2007. Obtenção de derivados da mistura triterpenoídica α-e β-amirina. Rev bras farmacogn, 204-208. http://dx.doi.org/10.1590/s0102-695x2007000200012.

Brasil; Anvisa., 2018. Agencia nacional de vigilância sanitária resolução da diretoria colegiada- rdc nº 26, de 13 de maio de 2014. Disponível em: <http://bvsms.saude.gov.br/bvs/saudelegis/anvisa/2014/rdc0026_13_05_2014.pdf> acessado em: 10 mai.

Bruneton, J. Burseraceae in flora do Brasil. Pharmacognosy, phytochemistry and medicinal plants. Ed. Lavosier, paris, 1993. Jardim botânico do Rio de Janeiro. Disponível em: <http://floradobrasil.jbrj.gov.br/reflora/floradobrasil/fb6593>. Acesso em: 13 mai. 2018.

Carneiro, A.B.A., Pinto, E.J.S., Ribeiro, I.F., Magalhães, M.R.G., Monteiro Neto, M.D.A.B. 2017. Efeito da Astrocaryum aculeatum (Tucumã) na toxicidade da Doxorrubicina: modelo experimental in vivo. Acta Paulista de Enfermagem, 30(3), 233-239.

Countryman, P.I.; Heddle, J.A. The Production of Micronuclei From Chromosome Aberrations In Irradiated Cultures Of Human Lymphocytes. Mutation res. 321-332, 1976.

De Azevedo Bentes Monteiro Neto, M., De Souza Lima, I.M., Furtado, R.A., Bastos, J.K., Da Silva Filho, A.A., Tavares, D.C., 2011. Antigenotoxicity of artepillin C in vivo evaluated by the micronucleus and comet assays. Journal of Applied Toxicology 31, 714-719. https://doi.org/10.1002/jat.1614

Dias, M.O., Hamerski, l., Pinto, A.C. 2011. Separação semipreparativa de α e β-amirina Por Cromatografia Líquida De Alta Eficiência. Quim. Nova, 704-706.

Ermolaeva, M.A.; Schumacher, B. 2014. Systemic DNA Damage Responses: Organismal Adaptations To Genome Instability. Trends in genetics, 95–102.

Fabiyi, O. A., Atolani, O., Adeyemi, O. S., Olatunji, G. A. 2012. Antioxidant and Cytotoxicity of β-amyrin Acetate Fraction From Bridelia Ferruginea Leaves. Asian Pacific Journal of Tropical Biomedicine, s981–s984. Doi:10.1016/s2221-1691(12)60347-5

Florentino N, S. 2018. Desenvolvimento E Caracterização Físico, Química E Biológica In Vitro De Nanopartículas Polimericas Contendo Α-Β Amirina.

Franke, S.I.R., Prá, D., da Silva, J., Erdtmann, B., Henriques, J.A.P. 2005. Possible repair action of Vitamin C on DNA damage induced by methyl methanesulfonate, cyclophosphamide, FeSO4 and CuSO4 in mouse blood cells in vivo. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 75-84.

Karam, T.K., Dalposso, L.M., Casa, D.D., De Freitas, G.B.L. 2013. Carqueja (Baccharis trimera): utilização terapêutica e biossíntese. Revista Brasileira de plantas medicinais, 15(2), 280-286. http://dx.doi.org/10.1590/s1516-05722013000200017.

Macgregor, J.T., Wher, C.M., Gould, D.H. 1980. Clastogen-Induced Micronuclei In Peripheral Blood Erythrocytes: The Basis Of An Improved Micronucleus Test. Environmental And Molecular Mutagenesis, V. 2, N. 4, P. 509-514.

Majone, F., Beltrame, C., Brunetti, R. 1988. Frequencies Of Micronuclei Detected On Mytilus Galloprovincialis By Differents Staining Techniques After Treatment With Zinc Chloride. Mutation Res. 131-134.

Matos, I., Bento, A.F., Marcon, R., Claudino, R.F., Calixto, J.B. 2013. Preventive And Therapeutic Oral Administration Of The Pentacyclic Triterpene Α, Β-Amyrin Ameliorates Dextran Sulfate Sodium-Induced Colitis In Mice: The Relevance Of Cannabinoid System. Molecular Immunology, 482–492. Doi: 10.1016/J.Molimm.2013.01.018

Mccaskill, D., Croteau, R. 1997. Prospects For The Bioengineering Of Isoprenoid Biosynthesis. Adv. Biochem. Eng. Biotechnol., 55: 107-146.

Mersch-Sundermann, V., Knasmüller, S., Wu, X., Darroudi, F., & Kassie, F. (2004). Use of a human-derived liver cell line for the detection of cytoprotective, antigenotoxic and cogenotoxic agents. Toxicology, 198(1-3), 329–340. doi:10.1016/j.tox.2004.02.009

Ministry of Health, 2009. Manual de diagnóstico laboratorial da malária. Série A. Normas e Manuais Técnicos. 2º ed. Ministério da Saúde, Brasília.

Neto, L.M.P. 2011. Avaliação Tóxica, Citotóxica, Genotóxica E Mutagênica Do Composto 3-(2-Cloro-6-Fluorobenzil)-Imidazolidina-2,4-Diona Em Células Eucarióticas. Dissertação De Mestrado, Universidade Federal Do Piauí.

Okoye, N.N., Ajaghaku, D.L., Okeke, H.N., Ilodigwe, E.E., Nworu, C.S., Okoye, F.B.C. 2014. Beta-Amyrin And Alpha-Amyrin Acetate Isolated From The Stem Bark Ofalstonia Booneidisplay Profound Anti-Inflammatory Activity. Pharmaceutical Biology,1478–1486. Doi:10.3109/13880209.2014.898078

Oliveira, L.S. De, Muzitana, M.F., Coutinho, M.A.S., Melo, G.O. De, Costa, S.S. 2011. Plantas Medicinais Como Recurso Terapêutico Em Comunidade Do Entorno Da Reserva Biológica Do Tinguá, Rj, Brasil – Metabólitos Secundários E Aspectos. Interscienceplace, v. 1, n. 17.

Punjab, N.W., Gul, F., Shimwari, Z.K.; Afzal, I. 2012. Screening Of Indigenous Knowledge Of Herbal Remedies For Skin Diseases Among Local Communities Of. Phoenix Usa. 1609–1616.

Ribeiro, L.R., Salvadori, D.M.F., Marques, E.K. 2003. Mutagênese Ambiental. Canoas: 1ºed. Ulbra.

Rodrigues, I.V. Estudo Biomonitorado Para Avaliação Antiinflamatória E Analgésica Das Folhas Da Espécie Protium Spruceanum (Benth) Engler. (Burseraceae). 2010. 121 F. Dissertação (Mestrado Em Ciências Farmacêuticas) - Escola De Farmácia Da Universidade Federal De Ouro Preto.

Sehmid, W. 1976. The Micronucleus Test For Cytogenetic Analysis. In: A. Hollaender (Ed.), Chemical Mutagens, Vol. 4, Plenum, New York, Pp. 31-53.

Silva, A.; Nepomuceno, J. 2010. Avaliação Da Frequência De Micronúcleos Em Eritrócitos Periféricos De Mandi-Amarelo (Pimelodus Maculatus) Do Rio Paranaíba. Revista Do Núcleo Interdisciplinar De Pesquisa E Extensão Do Unipam. (Issn 1806-6399). Patos De Minas: Unipam, N. 7, Vol. 1: 167-179.

Vale, E.P., Do Rego, L.R., Pureza, D.D.N., De Barros Silva, P.G., De Sousa, F.F.O. Neto, M.D.A.B.M., 2020. Cytogenetic And Toxicological Effects Of Punica Granatum Linnaeus Fruit Peel Hydroethanolic Extract In Mice. South African Journal Of Botany, 130, 465-470, Https://Doi.Org/10.1016/J.Sajb.2020.01.041

Venkatesh, P., Shantala, B., Jagetia, G.C., Rao, K., Baliga, M.S. 2007. Modulation Of Doxorubicin-Induced Genotoxicity By Aegle Marmelos In Mouse Bone Marrow: A Micronucleus Study. Integrative Cancer Therapies, 42-53.

Verpoorte, R. 2000. Pharmacognosy In The New Millennium: Leadfinding And Biotechnology. J. Pharm. Pharmacol., 52: 253-262.

Waters, M.D; Brady, A,L.; Stack, H.F.; Brockman, H.E. 1990. Antimutagenicity Profiles For Some Model Compounds. Mutation Research, V.238, P.57-85.

Zappi, D. C., Filardi, F. L. R., Leitman, P., Souza, V. C., Walter, B. M., Pirani, J. R., ... & Forzza, R. C. 2015. Growing knowledge: an overview of seed plant diversity in Brazil. Rodriguésia, 66(4), 1085-1113.

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Published

2021-04-01
CITATION
DOI: 10.31686/ijier.vol9.iss4.3007

How to Cite

Rego, L. R. do, Vale, E. P., Pureza, D. D. N., Neto, M. de A. B. M., Medeiros, F. A. de, & Martins, R. (2021). Cytogenetic evaluation of α, β-amirina obtained in resin of Protium heptaphylum (Aubl.) Marchand in polychromatics erythrocytes of mice swiss. International Journal for Innovation Education and Research, 9(4), 10–21. https://doi.org/10.31686/ijier.vol9.iss4.3007
Received 2021-02-19
Accepted 2021-03-01
Published 2021-04-01