Screening for antiviral activity of two purified saponin fractions of Quillaja spp. against Yellow Fever Virus and Chikungunya Virus

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Eduardo Troian
Karoline Schallenberger
Francini da Silva
Gabriela Dietrich
Fernando Ferreira Chiesa
Juliane Fleck
Simone Verza
Fraction B obtention
Fernando Ferreira


Yellow Fever Virus (YFV) and Chikungunya Virus (CHIV) are neglected reemerging pathogens that cause comorbidities worldwide. Since no antiviral drug is prescribed for those infections, there is a demand on researching compounds that inhibit viral replication. Saponins are amphiphilic compounds that already demonstrated in vitro activity against enveloped virus. Therefore, two purified saponin fractions from Quillaja spp. were evaluated regarding their antiviral potential against YFV and CHIKV. The cell line used in this study was VERO (African green monkey kidney cells) since it is permissive to the replication of both viruses. The antiviral activity of both saponins fractions was screened using the plaque reduction assay protocol. Although saponins did not inhibited YFV replication, they strongly inhibited CHIKV. To confirm the absence of antiviral activity of Quillaja saponins against YFV, the cytopathic effect inhibition assay was performed also. Further studies are required to determine the antiviral mechanisms involved in the CHIKV inhibition.


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Troian, E., Schallenberger, K., da Silva, F., Dietrich, G. ., Ferreira Chiesa, F., Fleck, J., Verza, S., obtention, F. B. ., & Ferreira, F. . (2020). Screening for antiviral activity of two purified saponin fractions of Quillaja spp. against Yellow Fever Virus and Chikungunya Virus . International Journal for Innovation Education and Research, 8(9), 205-214.
Author Biographies

Eduardo Troian, Feevale University

Graduate Program in Virology

Karoline Schallenberger, Feevale University

Graduate Program in Virology

Francini da Silva, Feevale University

Graduate Program in Virology

Gabriela Dietrich, Feevale University

Institute of Health Sciences


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